atf6 antibody - bsa free  (Bio-Techne corporation)

Journal: Scientific Reports

Article Title: AMPK-independent inhibition of human macrophage ER stress response by AICAR

doi: 10.1038/srep32111

Figure Lengend Snippet: ( A ) mRNA expression of ER stress markers, ( B , C ) Western analysis of cell lysates ( B ) or nuclear extracts ( C ).( D ) mRNA expression of ATF4 and ATF6α in macrophages pre-exposed for 1 h to AICAR and treated with thapsigargin or tunicamycin for 6 h. ( E ) Western analysis of macrophages pre-exposed for 1 h to 0.5 mM AICAR in the absence or presence of ABT-702 and treated with thapsigargin for 6 h. ( F ) mRNA expression of CHOP and Grp78 in macrophages pre-exposed for 1 h to indicated concentrations of AICAR in the absence or presence of ABT-702 and treated with thapsigargin or tunicamycin for 6 h. ( G ) Protein expression of AMPK 72 h post-transfection with control siRNA or AMPKα1 siRNA. ( H ) mRNA expression of CHOP, Grp78 and AMPKα1 in macrophages transfected with control siRNA or AMPKα1 siRNA for 72 h prior to treatments with thapsigargin for 6 h with or without 1 h AICAR pre-exposure. *p < 0.05. Data represent mean values ± SE of at least three independent experiments. ns, non-specific band. Western Blot images are cropped scans either of the same or of the duplicate membranes probed with different antibodies.

Article Snippet: Membranes were incubated with antibodies against phospho-ACC (Ser79) (#3661), phospho-AMPK (Thr172) (#2531), phospho-eIF2α (Ser51) (#3398), phospho-cJun (Ser73) (#3270), phospho-S6 ribosomal protein (Ser240/244) (#2215), ACC (#3662), AMPK (#2532) (Cell Signalling Technology), sXBP1 (6196, BioLegend), phospho-IRE1 (Ser724) (ab124945, Abcam), ATF6 (NBP1-75478, Novus Biologicals), ATF4 (sc-200), or nucleolin (sc-13057) (Santa Cruz Biotechnology) followed by IRDye 800-coupled secondary antibodies (LICOR Biosciences).

Techniques: Expressing, Western Blot, Transfection, Control